hSpCas9 IVT mRNA
VectorBuilder's hSpCas9 IVT mRNA can be ordered with or without the modified nucleotide, N1-Methylpseudouridine (m1Ψ) and its gene editing function has been functionally validated in cell culture.
Ordering Information Price Match
Click here to view the amino acid sequence translated from hSpCas9 mRNA >>
Shipping and storage
Our IVT mRNA products are stored in a 1 mM sodium citrate buffer (pH 6.4) and can be stored at -80°C for up to 12 months. The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Experimental Validation
Figure 1. Validation of hSpCas9 mRNA in vitro. (A) IVT Cas9 mRNA was transfected into 293T-EGFP cells with two types of EGFP-targeting gRNA. EGFP expression in non-treated (NC) and transfected cells was observed by microscopy (B) and quantified using flow cytometry (C + D). (E + F) The editing to EGFP genes on the genome was further confirmed by T7E1 assay and Sanger sequencing.
Documents
Brochures & Flyers
Certificate of Analysis (COA)
Material Safety Data Sheet (MSDS)