Rules for assigning names to vector components (e.g. promoters, ORFs or linkers)

  • Components from VectorBuilder's popular component collections: The component's common name is used.
    Example: CMV, EGFP, Neo

  • ORFs retrieved from VectorBuilder's gene-based databases: Gene symbol followed by RefSeq accession number or VectorBuilder ORF collection number in brackets is used as name. A species abbreviation in lower case is added in front of the gene symbol following the rules below:

    • Human, mouse and rat are assigned single-letter abbreviations “h”, “m” and “r”, respectively.
    • Many additional common species are assigned two-letter abbreviations. For example, cat is “ct”, chimpanzee is “ch”, and dog is “dg”.
    • Less common species use three-letter abbreviations of their scientific names, followed by an underscore. The first letter in the abbreviation corresponds to the first letter of the genus name, and the next two letters in the abbreviation correspond to the first two letters of the species name. For example, Drosophila melanogaster is “dme_”.
    Example of a human ORF from NCBI RefSeq collection: hRHO[NM_000539.3]
    Example of a mouse ORF from VectorBuilder ORF collection: mRho[ORF032112]
    Example of a Drosophila melanogaster ORF from NCBI RefSeq collection: dme_w[NM_057439.2]
  • Sequences pasted by user: User must define the name of their pasted sequence in order to add the sequence to the vector. User defined name will be shown in brackets. Please note that if the user pasted a sequence that has 100% sequence identity to a component in VectorBuilder’s databases, the component name will be automatically set to the official name of the VectorBuilder component.
    Example of a user pasted component: {MyGene}

  • ORF from VectorBuilder’s database that is edited by user: When an ORF retrieved from VectorBuilder’s ORF database has been edited by the user to introduce mutations, a * will be automatically added next to the official ORF name to indicate that the ORF sequence is mutated. The user can then choose to use this name or change it to a different name. If the user chooses to change the name, brackets will be added to indicate that the name is defined by the user. Please note that epitope tags added to an ORF through the Add Tag function are not considered as mutations. Example of an ORF from VectorBuilder’s database that is edited by user: hRHO[NM_000539.3]* for automatically assigned name, which can be changed by the user to another name such as {MyGene}